Journal: Journal of Cellular and Molecular Medicine

Article Title: Extracellular matrix protein laminin enhances mesenchymal stem cell (MSC) paracrine function through αvβ3/CD61 integrin to reduce cardiomyocyte apoptosis

doi: 10.1111/jcmm.13087

Figure Lengend Snippet: Placenta‐derived multipotent cell (PDMC)‐conditioned medium (CM) reduces the percentage of apoptotic cardiomyocytes. ( A ) PDMC‐CM can reduce mouse cardiomyocyte apoptosis. Mouse cardiomyocytes treated with TNF‐α (20 ng/ml) alone or mixed with PDMC‐CM or mouse cardiomyocyte CM (mCardio‐CM) were stained for annexin V and propidium iodine (PI) and analysed by flow cytometry. Representative graphs and quantitative results ( B ) are shown and presented as the mean ± S.E.M. of three independent experiments. ( C ) PDMCs secrete high levels of GRO‐α, HGF and IL‐8. ELISA was used to detect GRO‐α, HGF and IL‐8 in PDMC‐CM and fibroblast (WS1) CM. All results are shown as the mean ± S.E.M. of three independent experiments. * P < 0.05; ** P < 0.01; and *** P < 0.005 compared to the control; ### P < 0.005 compared to WS1‐CM.

Article Snippet: WS1, human foetal skin fibroblasts, were purchased from Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan) and were cultured as recommended in minimum essential medium (MEM; Gibco‐Invitrogen) with 10% FBS (Gibco‐Invitrogen).

Techniques: Derivative Assay, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Control